UNESP Laboratório de Controle Biológico de Pragas Florestais


Amanda Rodrigues de Souza

Degree: Postdoc.Amanda

Project title: Population surveys and biological aspects of Leptocybe invasa (Hymenoptera: Eulophidae) and its parasitoid Selitrichodes neseri (Hymenoptera: Eulophidae)

Abstract: This project aims to elucidate the biology of Leptocybe invasa and its parasitoid Selitrichodes neseri in Brazil. In addition, as part of this research, surveys were carried out to determine the diversity of gall wasp species, and their parasitoids and other associates in northeastern Australia. This work also seeks to identify new species of hymenopteran gall-inducers that may potentially represent a significant risk of introduction and establishment in eucalypt plantations in Brazil.


André Ballerini Horta

Degree: Postdoc.

Project title: Microbial control of Gonipterus platensis (Coleoptera: Curculionidae): efficiency of Bacillus thuringiensis strains and species of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae).

Abstract: The Gonipterus complex has been standing out in the global context of insect-pest control of the eucalyptus crop. The recent invasions of new territories, the severe damage caused to plantations and the reduction of efficiency of the control strategies traditionally adopted generate the need of research on alternatives to contain the advance of the populations of these insects. This project aims to evaluate the efficiency of two biological control agents in different niches of development of these insects: Bacillus thuringiensis strains against Gonipterus larvae in development on leaves and species of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) against imature stages in development in the soil.


Carolina Jorge

Degree: PhD.Carolina Jorge

Project title: Bioecology, biological control and plant resistance for the management of Leptocybe invasa (Hymenoptera: Eulophidae) in Eucalyptus spp. plantations.

Abstract: This project aims to improve the knowledge of the bioecology, population dynamics and distribution of the Eucalyptus gall-wasp Leptocybe invasa in Uruguay and Brazil. The use of the parasitoid Selitrichodes neseri for biological control of L. invasa in Uruguay will also be assessed. Spatial distribution of L. invasa and its parasitoid S. neseri in a Eucalyptus spp. plantation will be studied.  Plant resistance will be explored such as another strategy for the Eucalyptus gall-wasp management. The information obtained from the project will be the basis for creation of a biological control and plant resistance program for this Eucalyptus pest. I expect to develop a suitable methodology for monitoring and to predict the evolution of L. invasa infestations. Given that there is no adequate management methodology and there are no geographical barriers to prevent the passage of the Eucalyptus gall-wasp between Brazil and Uruguay, working in partnership for the surveillance, monitor and management of this and another forestry pests is required.

Duration: 08/2015 – 07/2018


Simone Graziele Moio Velozo

Degree: PhD.simone

Project title: Development of mycoinseticides for control of the bronze bug Thaumastocoris peregrinus (Hemiptera: Thaumatocoridae) in eucalypt forests.

Abstract: Considering the importance of Thaumastocoris peregrinus in eucalyptus plantations and the fact that biological control can be used as a control in the future, this project aims to bring new technologies for microbial control of bronze bug using entomopathogenic fungi found in epizootics. Substrate selection processes, conditions suitable for large scale production, the conditions for the final product storage, and product behavior under field conditions (Brazil) for this will be taken into account so that the viability and infectivity of the  fungi are maintained.

Project collaborators: Dr. Carlos F. Wilcken, Dr. José Cola Zanuncio, Dr. José Eduardo Marcondes de Almeida and Maurício Guimarães Domingues (intern – undergraduate student).

Maurício Guimarães Domingues


Duration: 03/2015 – 02/2018


Murilo Fonseca Ribeiro

Degree: MSc.

Project Title: Biology and parasitism of Anaphes nitens Girault (1928) (Hymenoptera: Mymaridae) in eggs of Gonipterus platensis Marelli (1926) (Coleoptera: Curculionidae)

Abstract: Gonipterus platensis is one of the main defoliators beetles of Eucalyptus in Brazil. Biological control is nowadays widely used as combat strategy for this pest, and the egg-parasitoid wasp Anaphes nitens is na important natural enemy used to control G. platensis. This project aims to assess the biology of this parasitoid under different temperatures, diets and photoperiods, The tritrophic interation among Eucalyptus, G. platensis and A. nitens will also be investigated. Additionally, the project aims the current situation of parasitism in the field, in regions attacked by the pest in Brazil.

Duration: 07/2015 – 07/2017

Project collaborators: Vinicius Franco de Camargo

Vinicius Franco de Camargo


Dan Ruiz Severi

Dan Ruiz SeveriDegree: Undergraduate

Project title: Populations dynamics of insects in Eucalyptus plantations

Abstract: This project aims to evaluate the populations dynamics of some of the most important pests of Eucalyptus species, including Glycaspis brimblecombei (Hem: Aphalaridae), Thaumastocoris peregrinus (Hem: Thaumastocoridae) and Leptocybe invasa (Hym: Eulophidae), as well as their natural enemies. Yellow sticky traps are used within Eucalyptus stands for monitoring in each 500 ha of forest. The evaluation of the samples is monthly and comprises the whole year.

Project collaborators: Lucas Nascimento Inoue, Luis Renato Junqueira and Matheus Antônio.

Duration: 2008 – 2016


Maurício Magalhães Domingues

Degree: Undergraduate

Project title: Effect of bioinseticides on the parasitism of Cleruchoides noackae (Hymenoptera: Mymaridae) in eggs of Bronze bug

Abstract: Among the forms of control of Thaumastocoris peregrinus Carpintero & Dellapé, 2006 (Hemiptera: Thaumastocoridae) one of the most promising methods used in Brazil is biological control, which can occur through the egg parasitoid Cleruchoides noackae Lin & Huber, 2007 (Hymenoptera: Mymaridae), which was imported from Australia, or through the use of entomopathogenic fungi. To verify the possible interference of this microorganism on the parasitoid, this project aims to quantify and evaluate the effects of two bioinsecticide based on Beauveria bassiana and Metarhizium anisopliae on the parasitoid C. noackae and also the effects on its parasitism in T. peregrinus eggs. In addition, the project will have as a chemical standard the phytosanitary product that has Bifenthrin as active ingredient, being this registered for the control of T. peregrinus under field conditions in Brazil.

Duration: 10/2016 – 09/2017

Project collaborators: Simone G. M. Velozo, Luciane K. Becchi, Amanda R. Souza, Dr. Carlos F. Wilcken.

Lab Technicians

Lab Technicians - Vanessa, Lorena e Fernanda

Vanessa, Lorena and Fernanda provide technical assistance across a range of projects in the Laboratório de Controle Biológico de Pragas Florestais.









Murici Carlos Candelária

Degree: PhD.Murici

Project title: Biological control of forest caterpillars: evaluation of parasitism by Trichospilus diatraeae and Palmistichus elaeisis (Hymenoptera: Eulophidae).

Abstract: This project aims to evaluate the biological control of Thyrinteina arnobia (Lepidoptera: Geometridae) with the pupal parasitoids Trichospilus diatraeae and Palmistichus elaeisis.  Parameters such as parasitoids released per unit area, dispersal ability, and other factors will be assessed.

Duration: 08/2013 – 06/2017


Barbara de Oliveira Puretz

Barbara PuretzDegree: MSc.

Project title: Biological parameters of the parasitoid Selitrichodes neseri (Hymenoptera: Eulophidae)

Abstract: This project aims to study some biological parameters and the potential of S. neseri in eucalyptus seedlings infested by L. invasa, an exotic pest of Eucalyptus in Brazil. This parasitoid was imported to Brazil in 2015. In laboratory, the rearing of L. invasa and S. neseri it is being maintained for early experiments. Field studies with S. neseri will also be carried out in eucalypt plantations.

Duration: 03/2015 – 06/2017

Collaborators: Amanda Rodrigues de Souza and Vanessa Carvalho


Luciane Katarine Becchi

Degree: MSc.

Project title: Biology of Cleruchoides noackae (Hymenoptera: Mymaridae) in eggs of Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae) under different temperatures, and dispersal of this parasitoid  in eucalypt plantations in Brazil.

Abstract: Thaumastocoris peregrinus is an important pest of Eucalyptus trees. While C. noackae has been used as a biological control agent for T. peregrinus, its biology under different temperatures has not been studied. This project aims to evaluate the biology of the egg parasitoid C. noackae under different temperatures, assessing the longevity of the adults and duration of the larval, egg and pupal stages. Additionally, dispersal capacity of C. noackae and rates of parasitism in the field will also be evaluated.

Duration: 03/2015 – 06/2017


Luís Renato Junqueira

RenatoDegree: MSc.

Project title: Estimating economic injury levels for Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae) in eucalypt forests in Brazil

Abstract: The economic injury level (EIL) of a pest is essential to support decision-making inside an Integrated Pest Management (IPM) program. In this context this work intends to estimate values of the EIL and the control threshold for T. peregrinus in eucalypt plantations. This work uses a twin-plot methodology to assess forest productivity under attacked and non-attacked conditions.

Project collaborators: Lucas Nascimento, André Carignato and Murici Candelária.

Lucas inoue

Lucas Nascimento

Duration: 03/2014 – 03/2016


Natalia Medeiros de Souza

NataliaDegree: MSc.

Project title: Gonipterus platensis (Coleoptera: Curculionidae) – assessment of infestations in Eucalyptus plantations, biological aspects and control

Abstract: This project aims to assess the occurrence of G. platensis outbreaks, some biological parameters and control strategies that might help to improve the management of this pest. Infestations of G. platensis in eucalypt plantations were assessed in relation to host species, intensity of attacks and management strategies adopted by growers. Assays have also been carried out to evaluate egg masses on the field, leaf consumption of larvae on some hosts, resistance of some hybrids and efficiency of commercial products against adults.

Project collaborators: Laboratory technicians and undergraduate interns

Duration: 03/2015 – 03/2016


Carolina Jordan

Carolina JordanDegree: Undergraduate

Project title: Selection and molecular characterization of Bacillus thuringiensis Berliner isolates effective against larvae of Gonipterus platensis Marelli (Coleoptera : Curculionidae ).

Abstract: This project aims to evaluate the insecticidal activity of a group of Bacillus thuringiensis strains against Gonipterus platensis, the main coleopteran eucalypt defoliator in Brazil. Previous bioassays showed the toxic activity of B. thuringiensis var. tenebrionis against first instar larvae of G. platensis. Research looking for strains with a similar toxic profile could show us new genes or new proteins that can be used in the integrated management of this insect pest.

Duration: 08/2015 – 06/2017

Project collaborator: André Ballerini Horta


Caroline Santos Fausto

Caroline FaustoDegree: Undergraduate

Project title: Levels of damage of Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae) in Eucalyptus seedlings under semi- field conditions.

Abstract: This project aims to analyze the levels of damage in relation to different insect instars in eucalypt seedlings in order to determine the best timing for control, and which of the instars and levels of infestation is more detrimental for forest productivity.

Duration: 10/2015-08/2016


Gabriella Camargo

Gabriella CamargoDegree: Undergraduate

Project title: Podisus nigrispinus (Hemiptera: Pentatomidae) predation capacity on nymphs and adults of Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae)

Abstract: Given the importance of the pest Thaumastocoris peregrinus to Eucalyptus plantations, biological control studies have been intensified in the last few years. The predator Podisus nigrispinus has a generalist habit, is found in several ecosystems predating different pests and can be considered a success for biological control in Brazil. The project aims to evaluate P. nigrispinus predation capacity on nymphs and adults of T. peregrinus to assess it’s potential as a biological control agent for this pest.

Duration: 03/2016 – 03/2017


Giovanne Serrau

Giovanne SerrauDegree: Undergraduate

Project title: Assessment of granulated insecticide baits efficacy in the control of Atta sexdens (Hymenoptera: Formicidae)

Abstract: The use of granulated baits is the most popular strategy in Brazilian planted forests in order to control leafcutting ants. The aim of this study is to compare the efficacy among four commercial brands of granulated baits in the control of Atta sexdens (Hymenoptera: Formicidae). One of these has a plant extract as active ingredient, one fipronil, and the last two sulfluramid. After the application, the baits were assessed in different categories regarding to loading and devolution by the ants. At the moment, the experiment is still ongoing to verify if the apparently controlled colonies will keep inactive until six months after the application.

Duration: 09/2015 – 12/2016

3 thoughts on “UNESP Laboratório de Controle Biológico de Pragas Florestais

  1. Dear Researchers

    Nowadays in Mexico we are employed at the project of biological control of Leptocybe invasa, specie recently found in our country, and initiate the steps for the introduction of a parasitoid. I want to now if it is feasible raise the parasitoid on branches cut and supported in blioclimatic chambers

  2. Dear Dr. Juan Manuel,

    We are studying L. invasa in last three years and we introduced the parasitoid Selitrichodes neseri from South Africa in last May.
    L. invasa has been reared only in Eucalyptus saplings (E. camaldulensis or hybrids with crossing with this species, p.e. E. grandis x E. camaldulensis). In branches are not possible keep the gall wasp and the parasitoid. We infested the saplings in greenhouses or open environment and after 2 or 3 months we offer to the parasitoid in lab conditions. The parasitoid life cycle is approximately 20 dys at 25oC. This technique was developed by researchers of University of Pretoria.

  3. native Megastigmus spp as parasitoids have been reported from S. America. Is any of you can tell me about the native host/s of these Megastigmus spp

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